沈金花,谭鸿浩,雷 骏.箬竹叶氯仿部提取物对小鼠气管平滑肌的舒张作用及其机理[J].中南民族大学学报自然科学版,2017,(3):27-30
箬竹叶氯仿部提取物对小鼠气管平滑肌的舒张作用及其机理
Relaxant Action of Chloroform Extracts from Leaves of Indocalamus tessellatus on Mouse Airway Smooth Muscle
  
DOI:
中文关键词: 箬竹叶氯仿部提取物  气管平滑肌  电压依赖型钙离子通道  非选择性阳离子通道
英文关键词: Chloroform extracts from leaves of Indocalamus tessellatus  airway smooth muscle  voltage-dependent calcium channel  non-selective cation channel
基金项目:国家自然科学基金资助项目(81170227);湖北省自然科学基金重点资助项目(2012FFA028)
作者单位
沈金花,谭鸿浩,雷 骏 中南民族大学 生命科学学院医学生物研究所武陵山区特色资源植物种质保护与利用湖北省重点实验室武汉 430074 
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中文摘要:
      使用高钾溶液(80 mmol/L KCl)和乙酰胆碱(100 μmol/L ACh)对小鼠离体气管环进行了预处理诱导气道异常收缩,进而研究了箬竹叶氯仿部提取物(CEIT)对气管的舒张作用及其机理.结果表明: CEIT能够抑制高钾和ACh诱导的预收缩,且呈剂量依赖性.此外,CEIT阻断了VDCCs电流,抑制了细胞外Ca2+内流.在ACh诱导的收缩反应中,不仅VDCCs 通道参与其中,还有其他离子通道的参与. CEIT能够抑制NSCCs 通道,从而抑制内钙释放.CEIT 能够通过阻断VDCCs通道和NSCCs通道抑制外钙内流和细胞内钙释放,从而舒张小鼠气管平滑肌.提示CEIT中的活性成分可以作为一种治疗气道异常收缩的药物.
英文摘要:
      To investigate whether chloroform extracts from leaves of Indocalamus tessellatus (CEIT ) could relax precontracted airway smooth muscle and its mechanism, 80 mmol /L KCl and 100 μmol /LACH were used to induce abnormal contraction of airway smooth muscle. Results: The precontracted mouse ASM could be relaxed by CEIT in a concentration-dependent manner. Besides, CEIT could block VDCCs to restrain Ca2+ influx. In the precontraction induced by ACh, there might be other channels involved except VDCCs. CEIT could block NSCCs to restrain the release of intracellular calcium. Conclusions: CEIT can relax the precontracted airway smooth muscle by blocking the VDCCs and NSCCs, which suppressed the cytoplasm Ca2+ increase. These data suggested that CEIT might be potential drugs for the treatment of abnormal airway smooth muscle contraction.
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