邓旭坤,江善青,穆 俊,段 欢,舒广文,陈旅翼.山楂多糖的成分测定及其单糖组分分析研究[J].中南民族大学学报自然科学版,2017,(3):52-56
山楂多糖的成分测定及其单糖组分分析研究
Content Determination of Polysaccharide in Hawthorn and Component Analysis of Its Monosaccharide
  
DOI:
中文关键词: 山楂多糖  含量测定  组分分析
英文关键词: polysaccharide in hawthorn  content determination  component analysis
基金项目:国家科技部“十二五”科技支撑计划资助项目(2012BAI27B06);国家自然科学基金资助项目(81073147)
作者单位
邓旭坤,江善青,穆 俊,段 欢,舒广文,陈旅翼 中南民族大学 药学院武汉 430074 
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中文摘要:
      目的:研究山楂多糖的总多糖含量及单糖组分构成. 方法:用苯酚-硫酸法测定山楂多糖中的中性多糖含量,用间羟基联苯法测山楂多糖中糖醛酸含量,用红外光谱法初步探究山楂多糖官能团,用气相色谱法分析山楂多糖中单糖组分. 结果:山楂多糖中中性多糖的含量为72.00%;糖醛酸的含量为14.98%;红外光谱分析表明山楂多糖可能为一种氨基多糖;气相色谱法分析得山楂多糖的单糖组成为木糖、核糖、阿拉伯糖、鼠李糖、果糖、葡萄糖、半乳糖,其质量分数分别为5.53%、13.85%、14.26%、9.29%、8.37%、19.91%、21.93%. 结论:该方法比较便捷,重复性好,可用于山楂多糖中单糖的组成成分分析.
英文摘要:
      Objective : To study the total content of polysaccharide in hawthorn and its monosaccharide components. Methods : The content of neutral polysaccharide in hawthorn polysaccharides was determined by phenol-sulfuric acid method, the content of uronic acid in polysaccharides was determined by m-hydroxydiphenyl method, the functional group of the polysaccharide was preliminarily explored by FT-IR, and the monosaccharide components were analyzed by GC. Results: The content of neutral polysaccharide in hawthorn polysaccharide was 72.00%, and the content of uronic acid was 14.98%. FT-IR indicated that polysaccharide in hawthorn was probably a amino-polysaccharide. GC showed that the monosaccharide components of polysaccharide in hawthorn included xylose, ribose, arabinose, rhamnose, fructose, glucose and galactose, whose mass fraction were 9.29%, 13.85%, 14.26%, 5.53%, 8.37%, 19.91% and 21.93%,respectively. Conclusion: These methods are convenient and fast with good repeatability, which can be applied to component analysis of monosaccharide for polysaccharide in hawthorn
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