张 蕊,司晓棠,白 兰,何 辉,覃 瑞.白细胞介素32通过5’-LTR抑制HIV复制[J].中南民族大学学报自然科学版,2017,(4):40-44
白细胞介素32通过5’-LTR抑制HIV复制
Suppression of HIV Replicaton by Interleukin-32 via 5'-LTR
  
DOI:
中文关键词: 白细胞介素32  人免疫缺陷病毒  长末端重复序列
英文关键词: interleukin-32(IL-32)  human immunodeficiency virus(HIV)  long terminal repeat(LTR)
基金项目:国家自然科学基金资助项目(81402668)
作者单位
张 蕊1,司晓棠1,白 兰2,何 辉2,覃 瑞1 1 中南民族大学 生命科学学院武汉4300742 武汉大学 生命科学学院武汉 430072 
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中文摘要:
      目的:初步探讨白细胞介素32(IL-32)抑制HIV病毒复制的机制.方法:利用荧光色素酶活性测定筛选抑制HIV复制最显著的IL-32亚型,通过构建HIV的LTR系列截断质粒和TAR茎环结构的系列点突变质粒,确定IL-32抑制HIV复制作用的位点.结果:在Hela、Jurkat、293T三个细胞系中,IL-32γ和IL-32ε两种亚型对HIV的抑制作用最显著.在Hela细胞系中证实IL-32可通过抑制HIV 5'-LTR的活性抑制HIV复制,确定TAR形成的茎环结构在此过程中起重要作用.IL-32与TAR结构相互作用的位点定位在TAR的环状和半环状处.结论:IL-32能抑制HIV复制,可作为HIV治疗的潜在手段.
英文摘要:
      Objective: To investigate the mechanism of interleukin 32 (IL-32) inhibition of HIV replication. Methods: The most significant IL-32 subtype of HIV replication was screened by luciferase activity assays. By constructing a series of HIV plasmids containing LTR truncated mutations and point mutations of TAR hairpin ,the site of IL-32 inhibition on HIV replication was determined. Results: In the three cell lines of Hela, Jurkat and 293T, IL-32γ and IL-32ε subtypes had the most significant inhibitory effects on HIV. In Hela cell line, it was confirmed that IL-32 could suppress HIV replication by inhibiting the activity of HIV 5'-LTR, with the hairpin structure formed by TAR played an important role in this process. The action site of the IL-32 interaction with the TAR structure was located at the ring and the half ring of TAR. Conclusion: Il-32 can inhibit HIV replication as a potential means of HIV treatment.
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