陈兰洲,汪 静,武艳芳,柯 檀,梁亚楠,刘思瑶.纤细席藻对重金属镍胁迫的响应研究[J].中南民族大学学报自然科学版,2017,(4):45-50
纤细席藻对重金属镍胁迫的响应研究
The Responses of Phormidium tenue on the Stress of Heavy Metal Nickel
  
DOI:
中文关键词: 纤细席藻    叶绿素荧光动态  抗氧化系统
英文关键词: Phormidium tenue  Ni  chlorophyll a fluorescence transients  antioxygen system
基金项目:国家自然科学基金资助项目(31370421);湖北省技术创新专项(重大项目,2016ACA162)
作者单位
陈兰洲1,汪 静1,武艳芳2,柯 檀1,梁亚楠2,刘思瑶1 1 武汉大学 资源与环境科学学院湖北省环境修复技术研究中心武汉 430072
2 武汉文科生态环境有限公司武汉 430000 
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中文摘要:
      实验研究了纤细席藻 Phormidium tenue 对重金属镍胁迫的光合生理响应及外源抗氧化剂的保护作用, 结果发现: 在不同浓度 Ni2+ 短期处理纤细席藻时,其荧光产量随处理时间先发生急剧下降, 随后有所回升; 荧光产量随着 Ni2+ 浓度的增加而明显降低,在 100 mg/L Ni2+ 浓度下荧光曲线的 O、 J、 I、 P 相完全消失, 光合活性(Fv/Fm) 丧失. 经抗氧化剂抗坏血酸(ASC) 和 N-乙酰半胱氨酸( NAC) 分别预处理的纤细席藻荧光产量有一定程度恢复, 且ASC 抗氧化作用相比 NAC 更加明显,但其保护作用均随着时间增加而逐渐减弱. 在不同浓度的 Ni2+长期胁迫时,1 d 内抑制作用显著,叶绿素 a、 光合活性及电子传递链光合参数(ET0/ABS、ET0/TR0、RC/CS0) 下降显著,SOD 活性升高. 实验结果表明: 镍对纤细席藻的光合生理活性具有较强的抑制作用,而外源抗氧化剂可以短期内有效缓解重金属镍胁迫造成的损伤,因此在吸附去除重金属时可有效提高藻类的吸附效率和循环利用.
英文摘要:
      The effects of nickel on Phormidium tenue, including photosynthetic activity, antioxidant enzymes and carbohydrate metabolism were investigated. It was found that fluorescence intensity decreased rapidly in the first 10 min after Ni2+ was added into the system, and partly recovered to the normal. Ni2+ contents could inhibit the fluorescence production of P. tenue and aggravated as the increase of Ni2+ concentration, even O-I-J-P phases of chlorophyll a fluorescence transients disappeared absolutely as well as Fv/Fm with 100 mg /L Ni2+ . The two antioxidants, ASC and NAC,were able to relieve the stress of Ni2+ on P. tenue, and ASC was better at protecting capacity, while the anti-oxidation effects decreased as extension of treatment time. The chlorophyll a content, Fv/Fm and photosynthetic parameters(ET0/ABS, ET0/TR0, RC/CS0) decreased significantly after Ni2+ stressing for 24 h, meanwhile SOD activity oppositely increased to defend against stress of Ni2+. The results indicated that Ni2+ had an inhibitory effect on photosynthesis of P. tenue, and antioxidants could relieve effectively the inhibition, which could increase the absorption efficiency and recycling utilization of algae as bio-adsorbents.
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