孙宏浩,梁玉芬,赵晓双,黄 玲,廖天作.生物素链霉亲和素联合免疫层析用于快速检测NT-proBNP[J].中南民族大学学报自然科学版,2018,(2):6-9,15
生物素链霉亲和素联合免疫层析用于快速检测NT-proBNP
Biotin Streptavidin System Combined Immunochromatography for Rapid Detection of NT-proBNP
  
DOI:
中文关键词: 生物素-链霉亲和素  荧光免疫层析  N末端脑钠肽前体  即时检验
英文关键词: biotin-streptavidin  fluorescence immunochromatography  NT-proBNP  POCTs
基金项目:国家自然科学基金资助项目( 21501054,21401051) ;湖北省自然科学基金重点资助项目(2014CFA080);湖北省教育厅优秀中青年科技创新团队(T201705);湖北省大学生创新创业训练计划资助项目( 201410500021)
作者单位
孙宏浩1,梁玉芬1,2,赵晓双1,黄 玲2,廖天作1 1湖北工业大学 生物工程与食品学院湖北省工业发酵协同创新中心发酵工程教育部重点实验室武汉430068
2 江西中医药高等专科学校 医学基础部抚州344000 
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中文摘要:
      为提高常规免疫层析技术的检测灵敏度,引入生物素-链霉亲和素系统,以荧光微球作为标记物,建立了一种新型快速定量检测NT-proBNP的方法.试验结果表明:新型试剂盒在0.02~30 μg·L-1范围能对NT-proBNP实现快速检测.在全血检测中,新型试纸条所测NT-proBNP浓度与雷度快速免疫分析仪定量检测结果具有很好的一致性.新型免疫层析试纸条大大提高了快速检测的准确性,可广泛用于床旁检验(POCTs) 等临床诊断阶段.
英文摘要:
      In order to improve the sensitivity of conventional immunochromatography, a novel rapid and quantitative assay for NT-proBNP was established by introducing biotin-streptavidin system and using fluorescent microspheres as a marker. The results showed that the new kit could detect NT-proBNP rapidly in the range of 0.02—30 μg·L-1. In whole blood test, the NT-proBNP concentration measured by the new test strip was in good agreement with the quantitative detection results of the RadioMeter AQT90 FLEX analyzer. The new immunochromatographic test strip greatly improved the accuracy of rapid test, and may be widely used in clinical diagnosis such as bedside test (POCTs).
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