徐 鑫,郭旭梦* ,刘松青,王晓婉,梁华兵,马克恩,韦明英.稻瘟病抗性基因 Pi63 启动子4 个构建体的表达及抗性分析[J].中南民族大学学报自然科学版,2018,(2):36-40
稻瘟病抗性基因 Pi63 启动子4 个构建体的表达及抗性分析
Expression and Resistance Analysis of the Rice BlastResistance Pi63 Gene Driven by Four Pi63 Promoter Constructs
  
DOI:10.12130/znmdzk.20180208
中文关键词: 水稻  稻瘟病抗性  Pi63 基因  表达分析
英文关键词: rice  rice blast resistance  Pi63 gene  expression analysis
基金项目:湖北省自然科学基金资助项目 (2018CFB476) ; 中央高校专项基金资助项目(CZP17089)
作者单位
徐 鑫,郭旭梦* ,刘松青,王晓婉,梁华兵,马克恩,韦明英 中南民族大学 生命科学学院武陵山区特色资源植物种质保护与利用湖北省重点实验室生物技术国家民委重点实验室武汉 430074 
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中文摘要:
      利用农杆菌介导法获得带有不同长度启动子区域的稻瘟病抗性基因 Pi63全长基因的转基因水稻, 并采用实时荧光定量 PCR方法鉴定了 T1 代阳性植株的基因拷贝数,进一步测定了单拷贝转基因植株在接种稻瘟病菌前后Pi63基因的表达量变化. 结果表明: 接种后 4 个分别带有不同长度启动子的 Pi63 转基因植物 T1 代中Pi63基因的表达量均升高,其抗病相关的顺式作用元件处于 P1 与 P2 中间的区域,P0 到 P1 的区域存在负调控元件. 此结果可为进一步揭示水稻稻瘟病抗性基因 Pi63 的分子机理奠定基础.
英文摘要:
      Agrobacterium-mediated rice transformation was performed to obtain transgenic plants which have the the full length of rice blast resistance gene Pi63 with different cis-acting elements. The copy numbers of target gene in T1 progeny were identified by qPCR. Those T1 plants with single copy number of target gene were inoculated with blast fungus and the expression of Pi63 gene was detected before and after inoculation. The results showed that all of the expression levels of Pi63 gene with the 4 different cis-acting elements were higher after inoculation. The differential expression level indicated that cis-acting elements response to inoculation is at the region between P1 and P2 and negative elements is at the region between P0 and P1. The results may lay the foundation for revealing the molecular mechanism of rice blast resistance gene Pi63
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