| 程国军，谢 婧，殷 杰，彭 杨.豌豆根瘤菌gshR基因的抗氧化和共生固氮作用[J].中南民族大学学报自然科学版,2018,(4):31-34 |
| 豌豆根瘤菌gshR基因的抗氧化和共生固氮作用 |
| Antioxidation and Symbiotic Nitrogen Fixation of gshRGene in Rhizobium leguminosarum |
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| DOI：10.12130/znmdzk.20180407 |
| 中文关键词: 豌豆根瘤菌 gshR基因 抗氧化功能 共生固氮 荧光定量RT-PCR |
| 英文关键词: Rhizobium leguminosarum gshR gene antioxida symbiotic fixation real-time qRT-PCR |
| 基金项目:湖北省自然科学基金资助项目( 2016CFC771) ; 中央高校基本科研业务费专项资金资助项目( CZW15112) |
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| 中文摘要: |
| 目的: 研究豌豆根瘤菌 RL3841 的谷胱甘肽还原酶编码基因 gshR 的功能．方法: 采用基因同源重组构建了豌豆根瘤菌 gshR 基因突变株 RLgshR，探讨了基因突变对根瘤菌抗氧化和共生固氮的影响， 利用实时荧光定量 RT-PCR检测 gshR基因的 mRNA 表达水平．结果: gshR基因缺失不影响菌株在 AMS 基础培养基中的生长能力， 但突变株对氢过氧化枯( CuOOH) 和较高浓度的 H2O2氧化物敏感．结论: gshR 基因突变株 RLgshR 形成正常固氮根瘤， gshR 基因的表达不受 H2O2和共生环境诱导． |
| 英文摘要: |
| Objective: To study the function of glutathione reductase gene gshR in rhizobium leguminosarum． Method: gshR
mutant RLgshR was constructed through homologous recombination， and the effect of gene mutation on its symbiosis
nitrogen fixation and antioxidative function were explored． Quantitative real-time RT-PCR was performed to detect mRNA
expression of gshR gene．Results: The growth ability of RLgshR in AMS basal medium was not affected by the lack of gshR
gene; however，the mutant strain was sensitive to CuOOH and high concentrations of H2O2． Conclusion: The gshR mutant
RLgshR formed nodules with normal nitrogen-fixing activity and gshR gene expression can not be induced under H2O2 stress
and symbiotic condition． |
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