阳小飞,荣伊,周仲燕.细胞内Ca2+对可释放囊泡库的影响[J].中南民族大学学报自然科学版,2019,(3):362-365
细胞内Ca2+对可释放囊泡库的影响
Effect of intracellular Ca2+ on readily releasable pool
  
DOI:10.12130/znmdzk.20190308
中文关键词: 1,2-双(2-氨基苯氧基)乙烷-N,N,N,N-四乙酸四(乙酰氧基甲酯)  大脑皮层神经元  囊泡释放  可释放囊泡库
英文关键词: BAPTA-AM  cerebral cortical neurons  vesicle release  RRP
基金项目:国家自然科学基金资助项目(31670850);中南民族大学科学基金引进人才科研启动基金自科项目(ZZ130020)
作者单位
阳小飞,荣伊,周仲燕 中南民族大学 生物医学工程学院脑认知国家民委重点实验室医学信息分析及肿瘤诊疗湖北省重点实验室膜离子通道与药物研发实验室武汉430074 
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中文摘要:
      目的:探讨细胞内Ca2+对可释放囊泡库的影响.方法:取体外培养成熟的新生鼠大脑皮层神经元细胞,分别对正常细胞、去除细胞外Ca2+的细胞、同时去除细胞内外Ca2+的细胞,记录微小型兴奋性突触后电流(mEPSCs)和微小型抑制性突触后电流(mIPSCs),观察兴奋性和抑制性突触可释放囊泡库(RRP)大小,研究细胞外和细胞内Ca2+对细胞胞吐的不同影响.结果:去除神经元细胞外Ca2+和细胞内Ca2+均对mEPSCs和mIPSCs的电流频率有抑制作用,细胞外Ca2+对RRP大小并无显著影响,而去除细胞内Ca2+可引起细胞内RRP的显著降低.结论:细胞内Ca2+可影响RRP的维持.
英文摘要:
      Objective: To study the effect of intracellular Ca2+ on readily releasable pool (RRP). Methods: Cerebral cortical neuron cells in mature neonatal rat were cultured in vitro. Spontaneous miniature excitatory postsynaptic currents (mEPSCs), miniature inhibitory postsynaptic currents (mIPSCs), excitatory and inhibitory RRP size in cultured cortical neurons with or without extracellular and intracellular Ca2+ were recorded, respectively. Results: The current frequencies of mEPSCs and mIPSCs were reduced in neurons lacking both the extracellular and intracellular Ca2+. The size of RRP was not affected by extracellular Ca2+, but was reduced significantly upon removing intracellular Ca2+. Conclusion: Intracellular Ca2+ may affect the maintenance of the RRP size of cortical neurons.
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