姜楠,吴云华.细胞色素P450 55B1的直接电化学及对一氧化氮的电催化检测[J].中南民族大学学报自然科学版,2020,39(3):245-249
细胞色素P450 55B1的直接电化学及对一氧化氮的电催化检测
Direct electrochemistry of cytochrome P450 55B1 and electrocatalytic detection of nitric oxide
  
DOI:10.12130/znmdzk.20200304
中文关键词: 细胞色素P450 55B1  一氧化氮;热解石墨电极;电化学传感器;一氧化氮还原酶
英文关键词: cytochrome P450 55B1  nitric oxide  pyrolytic graphite electrode  electrochemical sensor  nitric oxide reductase
基金项目:国家自然科学基面上资助项目(31670372);中央高校基本科研业务费专项基金资助项目(CZZ18004)
作者单位
姜楠 中南民族大学 生命科学学院武汉 430074 
吴云华 中南民族大学 生命科学学院武汉 430074 
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中文摘要:
      采用戊二醛(GA)交联法将细胞色素P450 55B1(CYP55B1)固定在热解石墨电极(PGE)表面,牛血清白蛋白(BSA)封闭多余活性位点,成功构建了一种新型电流型一氧化氮生物传感器,实现了对一氧化氮(NO)的检测. 并考察了电化学生物传感器的选择性与稳定性.结果表明:CYP55B1实现了直接电子传递,氧化还原峰电位位于?0.355 V和?0.385 V,CYP55B1对一氧化氮具有电催化活性,一氧化氮的催化还原峰位于?0.85 V. 一氧化氮的检测线性范围为14.4~108 μmol.L-1, 检测限为10.47 μmol.L-1,且该生物传感器具有良好的稳定性及选择性.
英文摘要:
      Cytochrome P450 55B1 (CYP55B1) was immobilized on the surface of pyrolytic graphite electrode (PGE) by glutaraldehyde (GA) crosslinking and bovine serum albumin (BSA) blocking the excess active sites. A new nitric oxide biosensor was successfully developed. The selectivity and the stability of the electrochemical biosensor were also investigated. The results showed that the direct electron transfer of CYP55B1 was realized and its oxidative and reductive peak potential was in ?0.355 V and ?0.385 V, respectively. CYP55B1 has good electrocatalytic activity to nitric oxide, and the catalytic reductive peak potential for nitric oxide was in ?0.85V. The linear range of nitric oxide detection was from 14.4 to 108 μmol.L-1 and the detection limit was 10.47 μmol.L-1.The biosensor possesses good stability and selectivity.
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