黄蓉,王滟,张宪栋,杨会祥,刘吉开.植物内生真菌来源的倍半萜对LPS诱导细胞炎症反应的抑制作用[J].中南民族大学学报自然科学版,2020,39(3):255-258
植物内生真菌来源的倍半萜对LPS诱导细胞炎症反应的抑制作用
Inhibition of LPS-induced cell inflammatory response by sesquiterpenoid derived from endophytic fungi
  
DOI:10.12130/znmdzk.20200306
中文关键词: 倍半萜  抗炎  植物内生真菌  RAW264.7细胞
英文关键词: sesquiterpenes  anti-inflammation  endophytic fungi  RAW264.7 cells
基金项目:国家自然科学基金资助项目(GZQ15015);中央高校基本科研业务费专项资金资助项目(CZQ19013)
作者单位
黄蓉 中南民族大学药学院民族药学国家级实验教学示范中心武汉430074 
王滟 中南民族大学药学院民族药学国家级实验教学示范中心武汉430074 
张宪栋 中南民族大学药学院民族药学国家级实验教学示范中心武汉430074 
杨会祥 中南民族大学药学院民族药学国家级实验教学示范中心武汉430074 
刘吉开 中南民族大学药学院民族药学国家级实验教学示范中心武汉430074 
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中文摘要:
      探究马铃薯内生真菌次生代谢产物中新型倍半萜类化合物TA的抗炎作用. 方法:脂多糖(LPS)刺激RAW264.7细胞建立体外炎症模型,采用噻唑蓝(MTT)法检测细胞存活率、Griess试剂测定细胞NO的产生量、二氯荧光黄双乙酸盐(DCFH-DA)法检测细胞内活性氧(ROS)水平、酶联免疫吸附法(ELISA)测定细胞培养上清液中肿瘤坏死因子-α( TNF-α) 、白细胞介素-1β( IL-1β)的释放量、蛋白免疫印迹法(Western-blot)检测一氧化氮合成酶(iNOS),环氧合酶(COX-2)蛋白表达量,从而评价化合物TA的抗炎活性. 结果:化合物TA对细胞无毒性,可剂量依赖性地抑制LPS刺激产生的NO,降低ROS水平,减少细胞炎症因子TNF-α 和IL-1b 的产生及下调iNOS,COX-2蛋白的表达量. 结论:马铃薯内生真菌次生代谢产物中新型倍半萜类化合物TA具有显著的抗炎活性.
英文摘要:
      Objective: To investigate the anti-inflammatory effect of a new sesquiterpene compound TA in the secondary metabolites of potato endophytic fungi. Methods: Lipopolysaccharide (LPS) stimulated RAW264.7 cells to establish an in vitro inflammation model. Cell viability was measured using thiazole blue (MTT) colorimetry, nitrite levels representing NO production were measured byGriess reagent, 2,7-dichlorodi-hydrofluorescein?diacetate (DCFH-DA) method was used to detect intracellular reactive oxygen species (ROS) levels.Enzyme-linked immunosorbent assay (ELISA) was usedto detect the release of inflammatory cytokines into the supernatant such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β).Moreover, Western-blottingassays were used to detect the expression of induced-nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins to evaluate the anti-inflammatory activity of compound TA. Results: Compound TA was non-toxic to cells, and could inhibit NOproduced, reduce ROS level, decrease the production of inflammatory cytokinesand down-regulate the expression of iNOS and COX-2 proteinsin a dose-dependent manner. Conclusion: TA, a novel sesquiterpene compound in the secondary metabolites of potato endophytic fungi, has significant anti-inflammatory activity.
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