王朝元,尹玉莹,魏天河,曾强,王鸣,王博.ELISA法对孕前孕中妇女及新生儿维普创免疫水平研究[J].中南民族大学学报自然科学版,2020,39(6):591-595
ELISA法对孕前孕中妇女及新生儿维普创免疫水平研究
Application of ELISA method to study immune status of Vptrmm in pre-pregnant, pregnant women and newborns
  
DOI:10.12130/znmdzk.20200607
中文关键词: ELISA  维普创  IgG抗体水平  孕前、孕中妇女及新生儿
英文关键词: ELISA  Vptrmm  level of IgG antibody  pre-pregnant and pregnant women and newborns
基金项目:病毒抗原制备及相关技术服务横向项目(HZY17059)
作者单位
王朝元 中南民族大学 生命科学学院武汉430074 
尹玉莹 中南民族大学 生命科学学院武汉430074 
魏天河 武汉武生医学检验实验室有限公司武汉 430073 
曾强 武汉武生医学检验实验室有限公司武汉 430073 
王鸣 武汉武生医学检验实验室有限公司武汉 430073 
王博 中国医科大学 附属盛京医院沈阳 110004 
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中文摘要:
      目的:通过检测孕前、孕期育龄妇女及新生儿“维普创”六项(水痘带状孢疹病毒VZV;百日咳类毒素PT;破伤风类毒素TT;风疹病毒RV;麻疹病毒MV和腮腺炎病毒MuV)的IgG抗体水平,为孕前、孕期育龄妇女及新生儿制定免疫策略及干预措施提供科学依据. 方法:以在中国医科大学附属盛京医院门诊进行孕前检查、孕中产前检查的妇女和新生儿人群作为调查对象,采静脉血分离血清,使用ELISA方法进行“维普创”六项IgG抗体定性和定量检测分析. 结果:检测人群VZV、PT、TT、RV、MV、MuV IgG抗体阳性率分别为75.08%、57.38%、43.28%、79.51%、90.66%和76.72%. 定量检测结果显示,6种IgG抗体滴度均处于中低水平. 孕前及孕期妇女VZV、RV、MV、MuV IgG抗体阳性率较高,但定量检测抗体滴度均处于中低水平. PT、TT IgG抗体阳性率较低. 结论:需进一步探讨和制定针对孕前、孕期妇女和新生儿进行相关疫苗强化免疫干预的可行性策略和措施.
英文摘要:
      Aim: By detecting the level of IgG antibodies of "Vptrmm" in the population of pre-pregnant, pregnant women and newborns to provide immune strategy and intervening measures for these people. Methods: Serums from blood collected from outpatients in Shengjing Hospital Affiliated to China Medical University were tested for IgG level of varicella-zoster virus VZV, Pertussis Toxoid PT, Tetanus Toxoid TT, Rubella RV, measles virus MV and Mumps virus MuV with ELISA. Results: ultThe positive rates of IgG to VZV, PT, TT, RV, MV and MuV were 75.08%, 57.38%, 43.28%, 79.51%, 90.66% and 76.72%, respectively. The results of quantitative analysis showed that the titers of 6 IgG antibodies were in the middle or low level. The positive rate of IgG antibodies of VZV, RV, MV and MuV in these people was high in pre-pregnant and pregnant women, but the titer of antibody was in the middle or low level. The positive rates of IgG for PT and TT were low. Conclution: It is necessary to further explore the feasible strategies and measures of vaccination for pre-pregnant, pregnant women and newborns.
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