周莉,杨富,张明浩,姚家旭.葡萄籽中原花青素定量方法的比较(英文)[J].中南民族大学学报自然科学版,2021,40(1):43-49
葡萄籽中原花青素定量方法的比较(英文)
Comparison of different methods for quantification of proanthocyanidins from grape seeds
  
DOI:10.12130/znmdzk.20210108
中文关键词: 葡萄籽  原花青素  定量  芳草醛-HCl测定法  Folin-Ciocalteu 测定法  酸性丁醇测定法
英文关键词: grape seed  proanthocyanidin  quantification  Vanillin-HCl assay  Folin-Ciocalteu method  acid butanol assay
基金项目:国家自然科学基金资助项目(31501521);中央高校基本科研业务费专项资金资助项目(CZY19032)
作者单位
周莉 中南民族大学 药学院&湖北省少数民族医学现代化工程技术研究中心武汉 430074 
杨富 中南民族大学 药学院&湖北省少数民族医学现代化工程技术研究中心武汉 430074 
张明浩 中南民族大学 药学院&湖北省少数民族医学现代化工程技术研究中心武汉 430074 
姚家旭 中南民族大学 药学院&湖北省少数民族医学现代化工程技术研究中心武汉 430074 
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中文摘要:
      研究了提取温度、时间、溶剂和提取次数对葡萄籽提取物中总多酚和三种主要酚类化合物提取产率的影响. 最佳提取条件为:温度50℃,乙醇水溶液80:20(乙醇/水,v/v)提取60分钟,连续提取4次. 此外,通过香草醛-HCl测定法和Folin Ciocalteu测定法与HPLC相结合,测定葡萄籽中原花青素的含量,并与酸性丁醇测定法进行了比较. 结果表明,香草醛HCl-HPLC法和酸性丁醇测定法比Folin-Ciocalteu-HPLC法更有效地定量原花青素,而香草醛HCl-HPLC和酸丁醇测定法则无显着差异(P>0.05). 同时,酸性丁醇测定法提供了一种简单,快速且有效的方法来定量原花青素, 可以更好地替代香草醛-HCl HPLC方法和Folin-Ciocalteu-HPLC方法来分析葡萄籽提取物或其他天然来源中的原花青素.
英文摘要:
      The effect of extraction temperature, time, solvent and the number of cycles on the solid-liquid extraction yield of total polyphenols and three main phenolic compounds in grape seed extracts were investigated. The optimal extraction conditions were obtained as follows: extraction temperature 50 ℃ and 60 min of extraction with 80:20 (ethanol/water, v/v) of aqueous ethanol for four successive extractions. Additionally, quantification of proanthocyanidins from grape seeds by vanillin-HCl assay and Folin-Ciocalteu assay in combination with HPLC was evaluated, and compared with acid butanol assay. The results showed that vanillin-HCl-HPLC method and acid butanol assay were more efficient than Folin-Ciocalteu-HPLC method for the quantification of proanthocyanidin, and there was no significant difference (P > 0.05) for vanillin-HCl-HPLC and acid butanol assay. Especially, acid butanol assay provides a simple, fast and effective method to quantify the proanthocyanidin, and could be a better alternative to vanillin-HCl-HPLC method and Folin-Ciocalteu-HPLC method on analyzing the proanthocyanidin in grape seed extracts or other natural sources.
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