生物素链霉亲和素联合免疫层析用于快速检测NT-proBNP
Biotin Streptavidin System Combined Immunoassay for Rapid Detection of NT-proBNP
投稿时间:2017-09-19  修订日期:2017-11-01
DOI:
中文关键词: 生物素-链霉亲和素  荧光免疫层析  NT-proBNP  POCTs
英文关键词: Biotin-streptavidin  Fluorescence immunochromatography  NT-proBNP  POCTs
基金项目:国家自然科学基金资助项目( 21501054,21401051) ,湖北省自然科学基金重点资助项目(2014CFA080),湖北省教育厅优秀中青年科技创新团队(T201705),湖北省大学生创新创业训练计划资助项目( 201410500021)
作者单位E-mail
孙宏浩 湖北工业大学 生物工程与食品学院湖北省工业发酵协同创新中心发酵工程教育部重点实验室 1048923282@qq.com 
梁玉芬 湖北工业大学 生物工程与食品学院江西中医药高等专科学校医学基础部  
赵晓双 湖北工业大学 生物工程与食品学院  
黄玲 江西中医药高等专科学校医学基础部  
廖天作 湖北工业大学 生物工程与食品学院  
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中文摘要:
      运用单一免疫层析技术检测NT-proBNP,由于在短时间内(15~30s),T线抗体不能有效对微量抗原-荧光微球复合体实现快速捕获,故在定量检测低浓度NT-proBNP(20~200ng/L)样品时存在较大误差。本文基于免疫层析技术,引入生物素-链霉亲和素系统,以荧光微球作为标记物,建立新型快速定量检测NT-proBNP的方法。结果表明,新型试剂盒能够对NT-proBNP实现快速检测,检测范围20ng/L~30000ng/L,这对于床旁检验(POCTs) 等临床诊断具有重要意义。
英文摘要:
      When NT-proBNP was detected by single immunochromatographic assay, the low concentration of NT-proBNP (20~200ng/L)could not be quantitatively detected because the antibody on the test line could not achieve rapid capture of the trace antigen-fluorescent microsphere complex in a short time (15~30s). Based on the immunochromatographic assay, a new method for rapid quantitative detection of NT-proBNP was established by combining the biotin-streptavidin system with fluorescent microspheres as a marker. The results showed that the new kit was able to achieve quantitative detection of NT-proBNP between 20ng/L and 30000ng/L, which was important for point-of-care tests (POCTs).
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