MTMR14稳定敲减细胞株的建立及表型分析
Establishment of MTMR14 stable knockdown cell line and phenotype analysis
投稿时间:2018-02-06  修订日期:2018-04-04
DOI:
中文关键词: CRISPR/Cas9  MTMR14  细胞增殖
英文关键词: CRISPR/Cas9  MTMR14  Cell Proliferation
基金项目:国家自然科学基金面上资助项目(31771274);中央高校专项基金项目(CZY18020)
作者单位E-mail
沈金花 中南民族大学生命科学学院 shenjinhua2013@163.com 
周婉 中南民族大学生命科学学院  
摘要点击次数: 189
全文下载次数: 
中文摘要:
      为了进一步研究MTMR14基因在肺癌细胞中的功能,我们运用CRISPR/Cas9基因编辑技术获得了基因敲减的人胚肾细胞株(293T),然后用筛选出的这一对具有编辑作用的sgRNA作用于腺癌人类肺泡基底上皮细胞(A549),获得基因敲减的A549细胞株,随后对野生型和敲减型细胞进行一系列表型分析。结果表明:MTMR14基因表达的减少能够促进细胞增殖。在非同步情况下,野生型和敲减型细胞周期没有明显变化。但敲减细胞中CyclinD1和CyclinE的rnRNA表达量显著升高,P21和P27的rnRNA表达量显著降低。
英文摘要:
      In order to further study the function of MTMR14 gene in lung cancer cells, we obtained a gene knockdown human embryonic kidney cell line (293T) by using the CRISPR / Cas9 gene editing technique and then used this pair of edited sgRNAs to act on adenocarcinoma human alveolar basal epithelium cells (A549) to obtain a gene knockdown A549 cell line, followed by a series of phenotypic analysis of wild-type and the knockdown cells. The results show that the decrease of MTMR14 gene expression can promote cell proliferation. In the absence of synchronization, there was no significant change in the cell cycle of WT and knockdown cells. However, the rnRNA expression levels of CyclinD1 and CyclinE in the knockdown cells were significantly increased, while the rnRNA expression levels of P21 and P27 were significantly decreased.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭