蒙药扎冲十三味丸抗风湿炎症性疾病的网络药理学研究
Study on Mechanism of Mongolian medicine Zhachong Shisanwei Pill for Anti-rheumatic Inflammatory disease Based on Network Pharmacology
投稿时间:2019-12-15  修订日期:2020-02-01
DOI:
中文关键词: 扎冲十三味丸  网络药理学  风湿炎症性疾病  靶点  通路
英文关键词: Zhachong Shisanwei pill  network pharmacology  rheumatic inflammatory disease  targets  pathway
基金项目:国家重点研发计划“蒙药经典方扎冲十三味丸和安神补心六味丸组方理论及临床定位研究”(No. 2018YFC1708200 & 2018YFC1708206)
作者单位E-mail
黄先菊 中南民族大学 xianju@mail.scuec.edu.cn 
董悦 中南民族大学  
王晶 中南民族大学  
佟海英 北京中医药大学 tonghaiying@sohu.com 
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中文摘要:
      目的:基于网络药理学方法探讨扎冲十三味丸中八种植物药抗风湿炎症性疾病的作用机制.方法:通过中药系统药理学分析平台(TCMSP)获取草乌、沉香、木香、甘草、丁香、诃子、肉豆蔻以及石菖蒲的化学成分,以口服生物利用度(OB)大于30%及药物相似度(DL)大于0.18为条件,结合文献研究筛选出以上8种植物药的主要活性成分;通过 TCMSP 及 Swiss Target Prediction 数据库查找筛选其活性成分的对应靶点.检索 OMIN等疾病靶点数据库,收集有关风湿炎症性疾病的靶点,映射至扎冲十三味丸活性成分靶点基因,构建活性成分-靶点网络,利用 STRING 网站构建共同靶点蛋白相互作用网络(PPI).将共同靶基因通过DAVID数据库进行 GO 功能富集与 KEGG 通路富集分析.结果:从扎冲十三味丸的8种植物药中筛选出93个活性成分,61个与抗风湿炎症性疾病相关的作用靶点;GO功能富集筛选得到生物学过程相关条目8条、分子功能相关条目3条及细胞组分相关条目6条;KEGG通路富集筛选得到18条相关通路,涉及癌症通路、破骨细胞分化信号通路等.结论:扎冲十三味丸通过TNF、破骨细胞分化等信号通路对细胞增殖、凋亡、炎症反应和机体的免疫调节等过程产生影响而发挥治疗风湿炎症性疾病的作用.
英文摘要:
      Objective: To explore the mechanism of the anti-rheumatic inflammatory disease effect of eight plant drugs in Zhachong Shisanwei pill based on the method of network pharmacology. Methods: The chemical components of Aconiti Kusnezoffii Radix, agarwood, Aucklandiae Radix, Glycyrrhizae Radix et Rhizoma,Caryophylli Flos, Terminalia chebula Retz, Myristica fragrants Houtt, Acori Tatarinowii Rhizoma were obtained by the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP) website. The main active ingredients of the above eight botanical drugs were screened under the condition of oral bioavailability (OB) more than 30% ,drug similarity (DL)more than 0.18 combined with related literatures. The corresponding targets of the active ingredients were screened by TCMSP and Swiss Target Prediction database. Searching the target database of OMIM and other diseases, the targets of rheumatic inflammatory diseases were collected, mapped to the target gene of the active ingredient of Zhachong Shisanwei pill, and the active ingredients-target network were constructed. Interactive protein-protein interaction network(PPI) were constructed by STRING website. The common target genes were subjected to gene ontology(GO) enrichment and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analyses by the David database. Results:93 active ingredients and 61 targets related to anti-rheumatic inflammatory disease were screened out from eight botanical drugs of Zhachong Shisanwei pill. 8 items related to biological process, 3 items related to molecular function and 6 items related to cell components were screened out by GO function enrichment; 18 related pathways related to cancer pathway and osteoclast differentiation signal were screened out by KEGG pathway enrichment, etc. Conclusion: Zhachong Shisanwei pill may play a role in the treatment of rheumatic inflammatory diseases through the influence of TNF signaling pathway, osteoclast differentiation signaling pathway on cell proliferation, apoptosis, inflammatory response and immune regulation.
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