应用ELISA方法对孕前孕中妇女及新生儿维普创免疫水平的研究
Application of ELISA Method to Study Immune Status of Vptrmm in Pre-pregnant, pregnant Women and Newborns
投稿时间:2020-06-16  修订日期:2020-07-28
DOI:
中文关键词: ELISA  维普创  IgG抗体水平  孕前、孕中妇女及新生儿
英文关键词: Elisa  Vptrmm  Level of IgG antibody  Pre-pregnant and pregnant women and newborns
基金项目:
作者单位E-mail
王朝元 中南民族大学生命科学学院 wangchaoyuan@mail.scuec.edu.cn 
尹玉莹 中南民族大学生命科学学院  
魏天河 武汉武生医学检验实验室有限公司  
曾强 武汉武生医学检验实验室有限公司  
王鸣 武汉武生医学检验实验室有限公司  
王博 中国医科大学附属盛京医院  
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中文摘要:
      通过检测孕前、孕期育龄妇女及新生儿“维普创”六项(水痘带状孢疹病毒VZV;百日咳类毒素PT;破伤风类毒素TT;风疹病毒RV;麻疹病毒MV和腮腺炎病毒MuV)的IgG抗体水平,为孕前、孕期育龄妇女及新生儿制定免疫策略及干预措施提供科学依据。以在中国医科大学附属盛京医院门诊进行孕前检查、孕中产前检查的妇女和新生儿人群作为调查对象,采静脉血分离血清,使用ELISA方法进行“维普创”六项IgG抗体定性和定量检测分析。结果显示:待检人群VZV、PT、TT、RV、MV、MuV IgG抗体阳性率分别为:75.08%、57.38%、43.28%、79.51%、90.66%和76.72%。定量检测结果显示,6种IgG抗体滴度均处于中低水平。孕前及孕期妇女VZV、RV、MV、MuV IgG抗体阳性率较高,但定量检测抗体滴度均处于中低水平。PT、TT IgG抗体阳性率较低。需进一步探讨和制定针对孕前、孕期妇女和新生儿进行相关疫苗强化免疫干预的可行性策略和措施。
英文摘要:
      By detecting the level of IgG antibodies of "Vptrmm" in the population of pre-pregnant, pregnant women and newborns to provide immune strategy and intervening measures for these people. Serums from blood collected from outpatients in Shengjing Hospital Affiliated to China Medical University were tested for IgG level of (varicella-zoster virus VZV, Pertussis Toxoid PT, Tetanus Toxoid TT, Rubella RV, measles virus MV and Mumps virus MuV) with Elisa. The result showed that the positive rates of IgG to VZV, PT, TT, RV, MV and MuV were 75.08%, 57.38%, 43.28%, 79.51%, 90.66% and 76.72%, respectively. The results of quantitative analysis showed that the titers of 6 IgG antibodies were in the middle or low level. The positive rate of IgG antibodies of VZV, RV, MV and MuV in these people was high in pre-pregnant and pregnant women, but the titer of antibody was in the middle or low level. The positive rates of IgG for PT and TT were low. It is necessary to further explore the feasible strategies and measures of vaccination for pre-pregnant, pregnant women and newborns.
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