七叶一枝花中尿苷二磷酸糖基转移酶基因的筛选和分析
Screening and analysis of Uridine diphosphate glycosyltransferase genes in Paris polyphylla var. Chinensis
投稿时间:2020-09-24  修订日期:2020-11-11
DOI:
中文关键词: 七叶一枝花  尿苷二磷酸糖基转移酶基因  qRT-PCR  序列分析  表达
英文关键词: Paris polyphylla var. Chinensis  UDP-glucuronosyltransferase gene  qRT-PCR  Sequences analysis  Expression
基金项目:中央高校基本科研业务费专项资金资助项目(CZY2003)
作者单位E-mail
宋发军 中南民族大学 生命科学学院 songfajun@scuec.edu.com 
李妍清 中南民族大学 生命科学学院  
杨瑞霜 中南民族大学 生命科学学院  
孟艳艳 中南民族大学 生命科学学院 yanzimeng1@163.com 
摘要点击次数: 134
全文下载次数: 0
中文摘要:
      重楼皂苷是重楼(Paridis rhizoma)中最重要的药用成分,但其生物合成途径仍不完全清楚。研究表明,尿苷二磷酸糖基转移酶(uridine diphosphate glycosyltransferase, UGT)是重楼皂苷生物合成下游步骤的关键酶。本研究利用生物信息学工具及qRT-PCR技术,对七叶一枝花(Paris polyphylla var. Chinensis)转录组数据库中UGTs的基因结构、系统进化、基因表达特征及其蛋白质理化性质和蛋白质结构等进行了分析,共得到45个UGTs基因,其主要分布在9个组及家族中;同时还获得了与重楼皂苷合成密切相关的UGT73和UGT80亚家族中的5个候选UGTs。表达量分析表明,5个候选UGTs多在叶或花中的表达量较高。研究结果为进一步探究UGT家族的基因功能,以及深入解析重楼皂苷合成途径提供了理论参考。
英文摘要:
      Polyphyllions are most important medicinal active ingredients in Paridis rhizoma, but their biosynthetic pathways are still unclear. Previous studies have shown that uridine diphosphate glycosyltransferase (UGT) are the key enzymes in the downstream steps of the polyphyllions biosynthesis. The gene structure, phylogeny, gene expression characteristics, protein physicochemical properties and protein structure of UGTs in the transcriptome database of Paris polyphylla var. Chinensis were studied by using the bioinformatics tools and qRT-PCR technology in this study. A total of 45 UGTs were obtained in the study, which mainly distributed in 9 groups and subfamilies. At the same time, 5 UGTs in UGT73 and UGT80 subfamily closely related to the synthesis of polyphyllions content were obtained as candidate genes. Analysis of expression levels showed that most of 5 candidate UGTs were highly expressed in leaves or flowers. The results provide a theoretical reference for further exploring the gene function of the UGT family and in-depth analyzing the synthetic pathways of polyphyllions.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭