汪文俊,王新宇,黄文伟,田薇,曾国祥.虾青素对体外脂多糖诱导下结直肠癌发生的影响[J].中南民族大学学报自然科学版,2022,41(4):406-412
虾青素对体外脂多糖诱导下结直肠癌发生的影响
Effects of astaxanthin against colorectal carcinogenesis by lipopolysaccharide challenge in vitro
  
DOI:10.12130/znmdzk.20220404
中文关键词: 虾青素;直肠癌;脂多糖;NF-kB  Nrf2
英文关键词: astaxanthin  colorectal cancer  lipopolysaccharide  NF-kB  Nrf2
基金项目:湖北省自然科学基金资助项目(2016CFB487);中央高校基本科研业务费专项资金资助项目(CZZ16003)
作者单位
汪文俊 中南民族大学 生命科学学院武汉430074 
王新宇 中南民族大学 生命科学学院武汉430074 
黄文伟 汉川市人民医院汉川432300 
田薇 中南民族大学 生命科学学院武汉430074 
曾国祥 汉川市人民医院汉川432300 
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中文摘要:
      为评估虾青素对脂多糖诱导条件下的癌症发生的保护作用,研究了脂多糖诱导条件下添加或不添加虾青素时,HCT116细胞 IL-6和IL1β的产生、细胞凋亡、caspase-3和caspase-9、NF-kB和Nrf2核转移及mRNA表达.结果表明:脂多糖诱导显著地增加了HCT116细胞中IL-6和IL1β的产生,而虾青素随添加量的增加明显抑制其产生.虾青素增加了HCT116细胞凋亡、caspase-3和caspase-9的表达,上调Nrf2的mRNA表达而下调NF-κB的mRNA表达.虾青素阻止了结直肠癌的发生和发展,并调节了NF-kB和Nrf2信号通路. 说明NF-kB和Nrf2信号通路可能在虾青素预防结直肠癌中有着重要的作用,而虾青素可能是一个新颖的化学预防与炎性相关癌症药物.
英文摘要:
      To evaluate the chemoprotective ability of astaxanthin (ASTA) against colorectal carcinogenesis after lipopolysaccharide (LPS) challenge in HCT116 cells. IL-6 and IL-1β secretion, apoptosis, caspase-3 and anti-caspase-9, as well as NF-kB and Nrf2 transference into nuclear, and mRNA expression of NF-kB and Nrf2 were investigated in HCT116 cells with or without ASTA treatment after LPS challenge. The results indicated that LPS challenge significantly increased IL-1β and IL-6 secretion in HCT 116 cells, while the secretion was remarkably inhibited by ASTA in a concentration-dependent manner. ASTA increased the percentage of apoptotic cells, increased expression of caspase-9 and -3, upregulated mRNA expression of Nrf2 and downregulated mRNA expressions of NF-kB in HCT116 cells. ASTA prevented the initiation and development of colorectal cancer (CRC) and regulated NF-kB and Nrf2 signaling pathways in HCT116 cells. These findings suggested NF-kB and Nrf2 signaling pathways might play a critical role for ASTA's prevention of CRC, and ASTA might be a new and attractive chemoprevention agent for inflammation-related cancer.
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