| 汪文俊,王新宇,黄文伟,田薇,曾国祥.虾青素对体外脂多糖诱导下结直肠癌发生的影响[J].中南民族大学学报自然科学版,2022,41(4):406-412 |
| 虾青素对体外脂多糖诱导下结直肠癌发生的影响 |
| Effects of astaxanthin against colorectal carcinogenesis by lipopolysaccharide challenge in vitro |
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| DOI：10.12130/znmdzk.20220404 |
| 中文关键词: 虾青素；直肠癌；脂多糖；NF-kB Nrf2 |
| 英文关键词: astaxanthin colorectal cancer lipopolysaccharide NF-kB Nrf2 |
| 基金项目:湖北省自然科学基金资助项目（2016CFB487）；中央高校基本科研业务费专项资金资助项目（CZZ16003） |
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| 中文摘要: |
| 为评估虾青素对脂多糖诱导条件下的癌症发生的保护作用，研究了脂多糖诱导条件下添加或不添加虾青素时，HCT116细胞 IL-6和IL1β的产生、细胞凋亡、caspase-3和caspase-9、NF-kB和Nrf2核转移及mRNA表达.结果表明：脂多糖诱导显著地增加了HCT116细胞中IL-6和IL1β的产生，而虾青素随添加量的增加明显抑制其产生.虾青素增加了HCT116细胞凋亡、caspase-3和caspase-9的表达，上调Nrf2的mRNA表达而下调NF-κB的mRNA表达.虾青素阻止了结直肠癌的发生和发展，并调节了NF-kB和Nrf2信号通路. 说明NF-kB和Nrf2信号通路可能在虾青素预防结直肠癌中有着重要的作用，而虾青素可能是一个新颖的化学预防与炎性相关癌症药物. |
| 英文摘要: |
| To evaluate the chemoprotective ability of astaxanthin (ASTA) against colorectal carcinogenesis after lipopolysaccharide (LPS) challenge in HCT116 cells. IL-6 and IL-1β secretion, apoptosis, caspase-3 and anti-caspase-9, as well as NF-kB and Nrf2 transference into nuclear, and mRNA expression of NF-kB and Nrf2 were investigated in HCT116 cells with or without ASTA treatment after LPS challenge. The results indicated that LPS challenge significantly increased IL-1β and IL-6 secretion in HCT 116 cells, while the secretion was remarkably inhibited by ASTA in a concentration-dependent manner. ASTA increased the percentage of apoptotic cells, increased expression of caspase-9 and -3, upregulated mRNA expression of Nrf2 and downregulated mRNA expressions of NF-kB in HCT116 cells. ASTA prevented the initiation and development of colorectal cancer (CRC) and regulated NF-kB and Nrf2 signaling pathways in HCT116 cells. These findings suggested NF-kB and Nrf2 signaling pathways might play a critical role for ASTA's prevention of CRC, and ASTA might be a new and attractive chemoprevention agent for inflammation-related cancer. |
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