榼藤子对组胺急性痒小鼠的治疗作用研究
The therapeutic effect of “Ke-Teng-Zi” on histamine-induced mice with acute itching
投稿时间:2022-04-19  修订日期:2022-06-29
DOI:
中文关键词: 榼藤子  榼藤  组胺  急性痒  炎性因子
英文关键词: Ke-teng-zi  Entada phaseoloides (Linn.) Merr  histamine  acute itching  inflammatory factor
基金项目:湖北省科技重大专项(2021ACA004);湖北省科技重大专项(2020ACA007)
作者单位邮编
鞠炎锟 中南民族大学 药学院 
和金凤 中南民族大学 药学院 
闫婷 中南民族大学 药学院 
熊慧 中南民族大学 药学院 430074
梅之南 中南民族大学 药学院 
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中文摘要:
      研究榼藤子提取物对组胺急性痒小鼠的治疗作用. 通过组胺诱导小鼠急性痒模型,将造模成功的小鼠随机分为模型组,榼藤子提取物低(25.0 mg/kg)、中(50.0 mg/kg)、高(100.0 mg/kg)剂量组,阳性药苯海拉明组(10.0 mg/kg). 结果表明榼藤子提取物可以降低组胺急性痒小鼠挠痒次数;明显改善皮肤表皮增厚;减少炎性细胞浸润;减少肥大细胞数量. 此外,榼藤子提取物可以下调皮肤中 H1R, TRPV1, IL-4, IL-6, IL-1β, IL-33, TNF-α, TSLP 以及 DRG 中痒相关因子受体的基因表达水平,降低皮肤中 H1R, TSLP 与 IL-4 的蛋白含量. 榼藤子提取物可以明显缓解组胺急性痒小鼠的症状,可能与其下调痒相关因子的基因与蛋白表达水平有关.
英文摘要:
      To study the therapeutic effect of “Ke-Teng-Zi” extract on histamine-induced mice with acute itching. The successful histamine-induced acute itch mice were randomly divided into model groups, “Ke-Teng-Zi” at 25.0, 50.0, and 100.0 mg/kg dosage groups, diphenhydramine was used as positive drug (10.0 mg/kg). The results showed that the extract of “Ke-Teng-Zi” could reduce the number of scratches in histamine-induced mice with acute itching; significantly improve skin epidermal thickening; relieved the infiltration of inflammatory cells and declined the number of mast cells. Furthermore, the extract of “Ke-Teng-Zi” could down-regulate the gene expression of HIR, TRPV1, IL-4, IL-6, IL-1β, IL-33, TNF-α, TSLP in skin and itch-related factor receptors in DRG, as well as decreasing the content of H1R, TSLP and IL-4 both in the skin tissue. The extract of “Ke-Teng-Zi” could significantly alleviate the symptoms of mice with acute itching induced by histamine, and the mechanism may be related to the down-regulation of gene and protein expression of itching factors.
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